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Beilstein J. Org. Chem. 2015, 11, 493–498, doi:10.3762/bjoc.11.55
Graphical Abstract
Scheme 1: Formation of the 2-aminobenzimidazole moiety.
Scheme 2: Synthesis of tris(2-aminobenzimidazole). Conditions: a: Boc-ON, THF, 0 °C to rt, 46 h, 45%; b: 1) 1...
Scheme 3: Synthesis of PNA conjugates. Conditions: a: 1) 9, HOBt, DIC, DMF, rt, 24 h; 2) piperidine, DMF, rt,...
Figure 1: Sequences of PNA conjugates 10–14 and oligonucleotides 15–20. Lysines are attached to the C-terminu...
Figure 2: Cleavage of RNA by their corresponding PNA conjugates (150 nM substrate, 750 nM conjugate, 50 mM Tr...
Figure 3: Substrate specificity of conjugates 12 and 14 (150 nM substrate, 750 nM conjugate, 50 mM Tris-HCl, ...
Figure 4: Cleavage of RNA substrates 15, 16, and 17 by their matching conjugates as a function of conjugate c...
Figure 5: Cleavage kinetics of 15 in the presence and absence of conjugate 12. Conditions: 150 nM substrate, ...
Beilstein J. Org. Chem. 2014, 10, 1495–1503, doi:10.3762/bjoc.10.154
Figure 1: (a) TAT triplet structure showing Watson–Crick and Hoogsteen base pairing; the binding can be reinf...
Scheme 1: Synthesis of the PNA monomer 1: i) 1. PPh3, H2O, THF; 2. TFA, 71%; ii) 1-pyreneacetic acid, HBTU, D...
Figure 2: Fluorescence spectra at 347 nm excitation, recorded at 20 °C of: (a) PNA1, (b) PNA2, (c) PNA3, (d) ...
Figure 3: (a) Increase in fluorescence intensity of the excimer band for PNA2 upon addition of complementary ...
Figure 4: Ratio of the intensities of the pyrene excimer (F474) and monomer emission (F379) for the PNA probe...